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Table 3 Distribution of exogenous mRNA in sub cellular foci

From: Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition

 

Mean number of red dots

Mean number of yellow dots

Stress granules

3h

18.4 (±5.9)

3.1 (±1.8)

18h

6 (±2.4)

1.5 (±0.8)

48h

2.6 (±0.9)

1.5 (±0.5)

P bodies

3h

10.8 (±4.6)

0

18h

8 (±2.8)

0

48h

1.7 (±0.7)

0

  1. Distribution of red fluorescent-labeled mRNA in green subcellular foci 3 h, 18 h and 48 h post-transfection. Yellow dots are obtained when there is collocation. Each value represents the average number of detected dots for each color ± standard deviation per whole cell. Three separate experiments were performed and at least 20 cells were analysed using confocal microscopy and ImageJ software for each condition.