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Table 3 Distribution of exogenous mRNA in sub cellular foci

From: Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition

  Mean number of red dots Mean number of yellow dots
Stress granules 3h 18.4 (±5.9) 3.1 (±1.8)
18h 6 (±2.4) 1.5 (±0.8)
48h 2.6 (±0.9) 1.5 (±0.5)
P bodies 3h 10.8 (±4.6) 0
18h 8 (±2.8) 0
48h 1.7 (±0.7) 0
  1. Distribution of red fluorescent-labeled mRNA in green subcellular foci 3 h, 18 h and 48 h post-transfection. Yellow dots are obtained when there is collocation. Each value represents the average number of detected dots for each color ± standard deviation per whole cell. Three separate experiments were performed and at least 20 cells were analysed using confocal microscopy and ImageJ software for each condition.