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Table 1 Distribution of mRNA polyplexes in three endocytosis compartments

From: Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition

 

Mean number of red dots

Mean number of yellow dots

Caveolae (Caveolin-1)

30m

9.3 (±3.5)

9.2 (±2.5)

2h

8.5 (±5.3)

6.3 (±3.6)

Clathrin-coated vesicle (Clathrin)

30min

4.4 (±1.7)

2.6 (±1.5)

2h

3.3 (±2.1)

2.3 (±1.5)

Late endosome (Rab-7)

30min

4.7 (±2)

1.1 (±0.4)

2h

7.2 (±2.6)

6 (±3.5)

  1. Red fluorescent-labeled mRNA polyplexes were observed in three green fluorescent endocytosis compartments 30 min and 3 h post-transfection. Yellow dots are obtained when there is colocalization. Each value represents the average number of detected dots for each color ± standard deviation per whole cell. Three separate experiments were performed and at least 20 cells were analysed using confocal microscopy and ImageJ software for each condition.
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