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Figure 6 | BMC Biotechnology

Figure 6

From: A streamlined implementation of the glutamine synthetase-based protein expression system

Figure 6

FcERα expression by stably transfected HEK 293S cells. (A). eGFP expression by sorted and unsorted FcERα-IRES-eGFP-Neo-pOPINEE12G transfected cells, one week after cell sorting. FcERα yield from these cells was four-fold lower than that of a clonal cell line (Neo clone 32), isolated previously by conventional limited dilution cloning. FcERα titre was determined by competition ELISA on supernatant samples collected from 175 cm2 flasks after three weeks (B). Error bars indicate standard errors for triplicate ELISA readings.

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