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Figure 3 | BMC Biotechnology

Figure 3

From: A streamlined implementation of the glutamine synthetase-based protein expression system

Figure 3

FcERα yields following cell sorting for different levels of eGFP expression. Triplicate transfections were performed and clones selected at 40 μM MSX. After three weeks, cells were removed with Accutase and sorted for the brightest 50%, 30% or 10% of the population. An unsorted population was also maintained. Flow cytometry was performed on these populations a week after the sort (A, gated on live cells), prior to populations being used to seed 175 cm2 flasks, which were treated with sodium butyrate upon confluency and left for three weeks before supernatant samples were collected. FcERα yield, determined by competition ELISA, shows the advantages of sorting for high levels of expression (B).

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