Efficiency of β-glucosidase amount on lactose hydrolysis in milk and analyzed by HPLC. A. Lactose hydrolysis was measured as a function of enzyme quantity (U/ml) at 65°C for 30 min using different concentrations of enzyme ranging from 1.7 U/ml to 498 U/ml as indicated. All data are presented as the mean ± SD of triplicate determinations. B. HPLC analysis of the reactions performed at 65°C for 30 min with 0 U/ml (Panel 1), 17 U/ml (Panel 2), 186 U/ml (Panel 3), or 498 U/ml (Panel 4) β-glucosidase enzyme. Lac = lactose, Glc = glucose, Gal = galactose.