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Figure 1 | BMC Biotechnology

Figure 1

From: Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiaeidentifies mutants with improved heterologous exocellulase activity and host secretion

Figure 1

Relative total activities of recombinant cellulases expressed in S. cerevisiae . (A) 4-methylumbelliferyl-β-D-cellobiose (4-MUC assay) for exocellulase activity with CBH I as a relative specific activity marker (100%). (B) Dye-carboxylmethy cellulose (dye-CMC) assay for endoglucanase activity with EgIII as the relative specific activity marker (100%). (C) p-nitrophenyl-b-D-glucopyranoside (pNPG) assay for beta-glucosidase activity with BglI as the relative specific activity marker (100%). The expression plasmid and related information are described in the Methods section. The 4-MUC, Dye-CMC and pNPG assay methods were as described in [21].

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BMC Biotechnology

ISSN: 1472-6750

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