Tender coconut water an economical growth medium for the production of recombinant proteins in Escherichia coli

BMC Biotechnology

Table 2 Chemical profile of TCW used in this study

Compounds	TCW 1 (μg/ml)	TCW 2 (μg/ml)	TCW 3 (μg/ml)	TCW 4 (μg/ml)	TCW 5 (μg/ml)	TCW 6 (μg/ml)	SD* (μg/ml)
Hydroxyproline	1.80	2.41	2.52	2.18	2.31	2.39	±0.23
Histidine	2.03	2.57	2.94	1.83	3.61	3.04	±0.60
Asparsgine	176.88	98.07	68.18	97.60	223.48	196.21	±57.92
Taurine	0.07	0.07	0.14	0.09	0.09	0.09	±0.02
Serine	8.27	8.03	8.40	7.01	11.27	10.36	±1.45
Glutamine	6.44	3.32	2.83	5.81	5.11	12.49	±3.17
Arginine	12.49	19.09	17.70	21.49	26.91	22.36	±4.43
Homoserine	1.30	1.39	1.06	1.16	1.31	1.33	±0.11
Glycine	2.90	2.78	2.80	2.39	3.00	3.98	±0.48
Aspartic acid	0.52	0.25	0.50	0.31	0.94	0.71	±0.23
Citrulline	1.02	1.37	1.20	1.10	1.29	1.23	±0.11
Glutamic acid	3.43	1.50	4.93	1.88	6.22	5.00	±1.71
Threonine	10.53	12.25	11.07	5.67	10.63	10.79	±2.08
Alanine	193.56	171.85	252.66	166.57	248.37	237.76	±35.71
Gaba	15.78	14.51	33.24	22.70	30.18	28.18	±7.07
Proline	20.78	56.54	50.26	17.23	31.98	34.73	±14.28
Lysine	0.92	1.27	1.96	1.50	2.14	1.71	±0.41
Tyrosine	6.69	3.48	4.27	4.00	10.32	9.02	±2.61
Methonine	0.03	0.04	0.06	0.16	0.09	0.11	±0.04
Valine	54.69	52.16	48.32	52.79	64.44	62.40	±5.74
Isoleucine	15.48	15.72	14.53	14.89	18.01	17.74	±1.34
Lucine	29.35	24.09	20.21	25.30	38.48	34.71	±6.28
Phenylalanine	7.28	5.03	5.07	4.80	8.66	7.81	±1.53
Total amino acids metabolites ¹	576.64	498.83	704.50	457.25	759.32	557.02	±107.19
Total carbohydrate ²	3.9 g/100 ml	3.82 g/100 ml	2.82 g/100 ml	2.80 g/100 ml	2.32 g/100 ml	2.63 g/100 ml	±0.611 g/100 ml
Total Nitrogen ³	33.2 mg/100 ml	17.542 mg/100 ml	33.60 mg/100 ml	15.0 mg/100 ml	57.2 mg/100 ml	28.630 mg/100 ml	±13.78 mg/100 ml

Amino acids and metabolites listed in the table were estimated using mass spectroscopy.
¹Sum of amino acid and other metabolites concentration estimated by mass spectroscopy.
²Total carbohydrate was estimated by phenol sulphuric acid method.
³Total nitrogen was estimated by Kjeldahl method.
*SD - standard deviation.

ISSN: 1472-6750