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Table 1 List of tested supplements: 1M stock solutions were prepared by dissolving the salts in water

From: Tender coconut water an economical growth medium for the production of recombinant proteins in Escherichia coli

Chemical name

Stock solution concentration

Final concentration

(NH4)2SO4

1 M

25 mM

Na2SO4

1 M

5 mM

KH2Po4

1 M

50 mM

MgSO4

1 M

2 mM

Glycerol

80%

0.8%

Glucose

40%

0.2%

Lactose

20%

0.5%

Amino acid mixture1 (− cys,-met,-tyr)

10 mg/ml each of 17 amino acids1

0.36%

  1. The above mentioned reagents were prepared as described by Neerathilingam and Markley [21].
  2. 1Amino acid cocktail without cystein, methionine and tyrosine was prepared.
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