Figure 5
Recombinant protein expression in E.coli grown in TCW media. Protein expression was analysed in 12% SDS-PAGE. (A) MBP-TEV protease expression at 69 KDa harvested after 5 hours (B) MBP expressed at 42 KDa harvested after 5 hours (C) mEos2 expressed at 27 KDa harvested after overnight induction. All samples normalised based on their OD600. Lane M: Marker, Lane U: Uninduced, Lane I: Induced. MBP & MBP-TEV constructs were induced with 0.4 mM IPTG while mEos2 with 0.1 mM IPTG.