Figure 2

Growth curve of E.coli and P.pastoris in TCW-S. Growth curve of (A) E.coli and (B) P.pastoris in TCW supplemented (TCW-S) with carbon sources (glucose, glycerol and lactose), (C) growth curve of E.coli and (D) P.pastoris in TCW-S with nitrogen and other components (25 mM NH₄SO₄, 0.36% amino acid cocktail, Na₂SO₄ 50 mM KH₂PO₄ and 2 mM MgSO₄). The experiments were performed in triplicates and SD has been incorporated.