From: Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA
DNase I treatment
RNA (ng/μL)*
Percentage of RNA recovered after each round¶
Round 0
247.2 ± 1.3
-
Round 1
130.3 ± 0.55
−47.3%
Round 2
84.2 ± 1.77
−65.9%
Round 3
52.3 ± 2.38
−78.8%