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Table 2 Quantification of total RNA extracted from a 48 h old liquid culture of Pseudomonas sp. LBUM300 after each round of DNase I treatment

From: Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

DNase I treatment

RNA (ng/μL)*

Percentage of RNA recovered after each round

Round 0

247.2 ± 1.3

-

Round 1

130.3 ± 0.55

−47.3%

Round 2

84.2 ± 1.77

−65.9%

Round 3

52.3 ± 2.38

−78.8%

  1. *: Avg of four replicates ± standard error of mean.
  2. : values in % with respect to original starting amount after each round of DNase-I treatment.