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Figure 3 | BMC Biotechnology

Figure 3

From: Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

Figure 3

PCR amplification of hcnC and phlD genes. Agarose gel electrophoresis of hcnC and phlD genes, PCR amplified from RNA (non-DNase I treated) extracted from liquid culture of Pseudomonas sp. LBUM300. Primer system: phlD-F/MYT4 (Lane 1-2); phlD-F/phlD-R: (Lane 3-4); hcnC-F/MYT4 (Lane 5-6) and hcnC-F/R: Lane (7-8). Template: Non-reverse Transcribed RNA (Lane 1, 3, 5, 7); cDNA (Lane 2, 4, 6, 8). M: Quick-Load DNA marker, Broad Range (New England Biolabs, Mississauga, ON).

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BMC Biotechnology

ISSN: 1472-6750

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