Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)

Table 1 Primer pairs and fluorogenic probes used for the conventional and real time-PCR

Primers and probes	Orientation	Sequence (5' →3')	Amplification length (bp)
Papain-A1	Forward	GGC TCA ATA TGG TAT TCA CTA CAG AAA T	363
Papain-A2	Reverse	CAT CGG TTT TGG CTG CAT AA
Coat protein-A1	Forward	GAC ATC TCT AAC ACT CGC GC	411
Coat protein-A2	Reverse	CTT CGA GAG CCA TAT CAG GTG
Papain-B1	Forward	AGT GGC TCA ATA TGG TAT TCA CTA CAG A	91
Papain-B2	Reverse	AAA ATG TAG ATA TAC CTC CCT TGA GCG
Papain-P	Probe	(FAM)-ATA CTT ACC CAT ATG AGG GAG TGC AAC GTT ATT G-(TAMRA)
Coat protein-B1	Forward	CCG CGG TAT GGA ATC AAG AG	100
Coat protein-B2	Reverse	TCG AGA GCC ATA TCA GGT GTT TT
Coat protein-P	Probe	(FAM)-CTC GCT AGA TAT GCT TTC GAT TTC TAT GCG GT-(MGB)

ISSN: 1472-6750