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Figure 3 | BMC Biotechnology

Figure 3

From: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)

Figure 3

Real-time PCR amplification of papain and coat protein (CP) primer pairs. A) ‘Waimanalo’. B) ‘Rainbow’. C) ‘SunUp’. Lanes in A, B and C depicting papain amplification, 1: water; 2–5: 100 ng/μl, 10 ng/μl, 1 ng/μl and 0.01 ng/μl of DNA respectively. D) Real-time PCR amplification of CP under different total nucleic acid concentrations of 100 ng/μl, 10 ng/μl, 1 ng/μl and 0.01 ng/μl (Lane 1: water; 2–5: ‘Rainbow’; 6–9: ‘SunUp’). M: 1 kb DNA ladder (Fisher Scientific).

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