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Figure 2 | BMC Biotechnology

Figure 2

From: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)

Figure 2

Conventional PCR amplification. A) Papain primer pair (Lanes 1,5: water; 2–4: papain PCR primer pair for ‘Rainbow’, ‘SunUp’ and ‘Waimanalo’ respectively; 6–8: papain probe for ‘Rainbow’, ‘SunUp’ and ‘Waimanalo’ respectively. B) Coat protein (CP) primer pair (Lane 1: water; 2–3: CP PCR primer pair for ‘Rainbow’ and ‘SunUp’ respectively; 4–5: CP probe for ‘Rainbow’ and ‘SunUp’ respectively. C) Papain primer pair (Lane 1: ‘Waimanalo’; 2–6: ‘SunUp’:‘ Waimanalo’ seed mixtures in the ratio of 10, 1, 0.4, 0.2 and 0.1%). D) CP (Lanes 1: ‘Waimanalo’; 2–6: ‘SunUp’: ‘Waimanalo’ seed mixtures in the ratio of 10, 1, 0.4, 0.2 and 0.1%). M: 1 kb DNA ladder (Fisher Scientific).

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