Skip to main content

Table 2 Primer sequences and qRT-PCR conditions

From: The effects of statins on the mevalonic acid pathway in recombinant yeast strains expressing human HMG-CoA reductase

Gene

Primer sequence

Annealing (°C)

Product size (bp)

ERG10

F: 5′-GTCTGTGCATCCGCTATGAA-3′

61

124

R: 5′-CTGCTGGCATGTAGTATGGT-3′

ERG13

F: 5′-TGGTAGAGACGCCATTGTAG-3′

61

153

R: 5′-GCGTGTTCCATGTAAGAAGC-3′

HMGR

F: 5′-ATGCTCACAGTCGCTGGATA-3′

61

199

R: 5′-ACAGCCAGAAGGAGAGCTAA-3′

HMG1

F: 5′-CCGTATCCATGCCATCCATC-3′

61

158

R: 5′-GACGGCACAGGCAACTATTC-3′

HMG2

F: 5′-CGCCATGCTTGATCTTCTCG-3′

61

121

R: 5′-GGAGCACAGAGACAGTTCAC-3′

FPP1

F: 5′-TACAACACTCCAGGCGGTAA-3′

61

166

R: 5′-CATCGGCGACCAAGAAGTAA-3′

ERG1

F: 5′-GTGTTATCGGTGACGCTCCTA-3′

61

122

R: 5′-TTCACGGTCGCTGAAGTCTA-3′

ERG6

F: 5′-AAGACCTGGCGGACAATGAT-3′

61

197

R: 5′-AGCAGCAGTAACTTCCTTGG-3′

ERG3

F: 5′-TCACGGCTAGTCTCAGCTAC-3′

61

134

R: 5′-AACGGTCAACATCGACATCC-3′

BTS1

F: 5′-TAGGGGACAAGGCTTGGATA-3′

61

168

R: 5′-ACCAACGAATGGCCGTGG TG-3′

COQ2

F: 5′-GTGTCTCGGCTGCCTAAGAA-3′

61

198

R: 5′-GCCAGCACCTCTCATTACCA-3′

COQ3

F: 5′-AGTGAGCGTCTTGGATGTTG-3′

61

183

R: 5′-TCCAGAGCCTTGCACTCATA-3′

CAT5

F: 5′-TGGCTTGTACTGAAGCTGTC-3′

61

195

R: 5′-CATGCTTGATAGCGGTGTCT-3′

RER2

F: 5′-GTCGATACGGCTACCGTGTT-3′

61

133

R: 5′-ACTTACAGGCCAGCTCTCCA-3′

SEC59

F: 5′-AAGGTATGGCCGCATTCGTT-3′

61

176

R: 5′-CTAGCACTCCACTCAGTGTA-3′

35S rRNA

F: 5′-TCGACCCTTTGGAAGAGATG-3′

60

203

R: 5′-CTCCGGAATCGAACCCTTAT-3′

  1. F, R forward and reverse primers, respectively.