Figure 4

Statins affect levels of human HMGR and yeast Rer2 and Coq3 proteins. Strains expressing GFP-HMGR, HA-Rer2 or Coq3-HA proteins were grown for 24 hours in the presence of buffer (B) or one of the statins: atorvastatin (A), fluvastatin (F), rosuvastatin (R) or simvastatin (S). Protein extracts were prepared and analyzed by Western blotting. The level of plasma membrane ATPase Pma1p is shown as a loading control. A) Steady state level of human HMGR is higher after statin treatment with the highest increase after rosuvastatin treatment. Western blot was analyzed with anti-GFP or, for loading control, anti-Pma1 antibody. Ratio of respective signals is shown below. B) Levels of yeast Rer2 and Coq3 proteins are marginally affected by statin treatment. Western blots were analyzed with anti-HA or, for loading control, anti-Vma2 antibody.