Figure 3
Statins induce expression of genes encoding enzymes of sterol and nonsterol biosynthesis pathways. mRNA levels in statin-treated cells relative to control (buffer-treated cells) are shown using log2 scale. Results are mean ± SEM obtained by qRT-PCR. *p < 0.05, ***p < 0.001. Real-time PCR data, were normalize to 35S rRNA, a housekeeping gene. S – simvastatin, A – atorvastatin, F – fluvastatin, R – rosuvastatin. A) Quantitative real-time RT-PCR analysis of selected genes encoding enzymes of sterol biosynthesis pathway after statin treatment compared to buffer-treated cells. ERG10 – acetyl-CoA acetyltransferase, ERG13 – 3-hydroxy-3-methylglutaryl-CoA synthase, HMGR – human 3-hydroxy-3-methylglutaryl-Co A reductase, HMG1 and HMG2 – yeast 3-hydroxy-3-methylglutaryl-Co A reductase 1 and 2, FPP1 – farnesyl pyrophosphate synthase, ERG1 – squalene monooxygenase, ERG6 – delta(24)-sterol C-methyltransferase, ERG3 – C-5 sterol desaturase. B) Quantitative real-time RT-PCR analysis of genes from ubiquinone and dolichol synthesis and protein prenylation pathways. CAT5 – ubiquinone biosynthesis monooxygenase, COQ3 – 3,4-dihydroxy-5-hexaprenylbenzoate-O-methyltransferase, COQ2 – para-hydroxybenzoate-polyprenyl transferase, BTS1 – geranylgeranyl diphosphate synthase, RER2 – cis-prenyltransferase, SEC59 – dolichol kinase.