Assessing the bioconfinement potential of a Nicotianahybrid platform for use in plant molecular farming applications

Table 3 Results of crosses made in the greenhouse to examine the sexual compatibility of hybrid GFP plants and the fertility of their progeny

Genotypes^a	Plants crossed^b	Total crosses^c	Crosses resulting in seed production	Total seed count	Seeds that germinated	Surviving plants
(A) F1 crosses
(hybrid GFP × SN 2108)^d	12:12	95	7	12	5	3
(MS TN 90 × hybrid GFP)^e	12:12	96	9	445	2	2
(hybrid GFP × hybrid GFP)	1:1	10	0	0	0	0
(B) Backcrosses
(MS TN 90 × HYB BC₁F₁)	2:1	60	0	0	0	0
(MS BC₁F₁ × NT-glauca)	1:1	20	2	128	74	74
(MS BC₁F₁ × NT-TN 90)	1:1	20	0	0	0	0
(MS BC₁F₁ × hybrid GFP)	1:1	20	0	0	0	0

^aCrosses listed by (female ♀ × male ♂).
^bRefers to numbers of each plant used in crosses with respect to the plant order in the cross column.
^cA ‘cross’ is constituted by a pollen transfer from one plant to the flower of another plant.
^dProgeny of this cross formed the individuals named HYB BC₁F_1.
^eProgeny of this cross formed the individuals named MS BC₁F₁.
Progeny generated from the initial hybrid GFP crosses were used in a subsequent set of crosses to test sexual compatibility of potential volunteers with other Nicotianas. The crosses shown in the top-most rows each for (A) F₁ crosses and (B) Backcrosses, produced progeny that were subsequently used for additional crosses. See Table 1 for plant nomenclature.

ISSN: 1472-6750