Sequence alignment of the CAD family of Brachypodium distachyon and other species. Amino acid sequence comparison of seven B. distachyon CAD proteins with other functionally characterized CAD proteins in Sorghum bicolor (Sbbmr6), Saccharum officinarum (SoCAD), Zea mays (Zmbm1), Panicum virgatum (PviCAD2), Oryza sativa (OsCAD2), Festuca arundinacea (FaCAD), and Triticum aestivum (TaCAD). The Zn-1 binding domain motif is labeled in pink and Zn-1 catalytic residues are labeled in yellow. The NADP(H) cosubstrate-binding domain motif is labeled in red and the Zn-2 structural motif is labeled in green. Substrate-binding residues are labeled in blue, and the Ser212 specific NADP(H) binding residue is labeled in purple.