Bni5 interacts with septins. A. Spin down assay: SDS-PAGE analysis of supernatant and pellet fraction after centrifugation of a sample containing Bni5-SNAP together with septin filaments (low salt (LS); left panel) or together with septin rods (high salt (HS); right panel). Bni5-SNAP co-sedimentation with the septins indicates interaction. B. Validation of the spin down assay with Western blot after SDS-PAGE using an anti-his antibody. 6his tagged Bni5-SNAP is exclusively detected in the pellet with the septins (6his-Cdc12 as control).