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Figure 3 | BMC Biotechnology

Figure 3

From: An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitroexperimentation

Figure 3

Bni5 interacts with septins. A. Spin down assay: SDS-PAGE analysis of supernatant and pellet fraction after centrifugation of a sample containing Bni5-SNAP together with septin filaments (low salt (LS); left panel) or together with septin rods (high salt (HS); right panel). Bni5-SNAP co-sedimentation with the septins indicates interaction. B. Validation of the spin down assay with Western blot after SDS-PAGE using an anti-his antibody. 6his tagged Bni5-SNAP is exclusively detected in the pellet with the septins (6his-Cdc12 as control).

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BMC Biotechnology

ISSN: 1472-6750

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