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Table 2 Effect of plasmid-based overexpression of genes on lycopene production in the CIChE strain E. coli CBW12241 *

From: Chromosomal evolution of Escherichia coli for the efficient production of lycopene

Plasmid

OD600

Lycopene concentration (mg/L)

Specific lycopene content (mg/gDCW)

pBAD24

9.54 ± 0.73

57.79 ± 1.60

19.01 ± 1.64

pBappY

8.95 ± 0.08

57.75 ± 0.49

20.16 ± 0.18

pBidi

10.20 ± 0.22

62.49 ± 1.43

19.15 ± 0.66

pBpck

10.28 ± 0.46

58.87 ± 2.34

17.90 ± 0.15

pBpps

10.51 ± 0.57

60.11 ± 0.97

17.91 ± 1.29

pBrpoS

10.91 ± 0.34

60.17 ± 1.54

17.25 ± 0.99

pBycgW

10.53 ± 0.42

60.43 ± 0.92

17.96 ± 0.54

pByjiD

10.54 ± 0.10

58.29 ± 0.34

17.28 ± 0.07

pBdxs

10.25 ± 0.45

62.02 ± 1.66

18.91 ± 0.47

pQE30

7.72 ± 0.13

52.92 ± 1.53

21.42 ± 0.67

pQwrbA

13.76 ± 0.47

7.51 ± 0.58

1.71 ± 0.13

pQydeO

13.19 ± 0.17

9.81 ± 0.62

2.33 ± 0.15

pQatpE

9.73 ± 0.17

45.21 ± 0.67

14.53 ± 0.06

pQdxs

11.49 ± 0.31

25.62 ± 1.84

6.97 ± 0.64

  1. a Cells were cultured in SBMSN medium supplemented with 5 g/L KAc without triclosan at 37°C for 48 h. Data represent means of triplicate cultures ± standard deviation.