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Table 1 Genetic stability of the CIChE and the plasmid-carrying strain a

From: Chromosomal evolution of Escherichia coli for the efficient production of lycopene

Number of subculture

OD600

Specific lycopene content (mg/gDCW)

E. coli CBW12241

1

6.85 ± 1.83

28.57 ± 0.50

5

5.59 ± 0.31

29.76 ± 0.76

10

4.37 ± 0.49

31.05 ± 1.11

15

4.47 ± 0.37

30.37 ± 1.21

20

4.61 ± 0.45

30.95 ± 1.21

25

4.39 ± 0.39

28.95 ± 0.85

30

4.92 ± 0.10

30.53 ± 1.53

E. coli BW25113 (Δ gdhAΔaceE, PT5-dxs, pBAD24-WZM1)

1

4.29 ± 0.51

7.51 ± 0.76

30, with Amp

7.15 ± 0.38

2.24 ± 0.46

30, without Amp

8.51 ± 0.45

0

  1. a Cells were cultured in SBMSN medium supplemented with 5 g/L KAc without triclosan at 37°C for 48 h. Data represent means of triplicate cultures ± standard deviation.