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Table 1 Putative proteins in the supernatant of a U. maydis MB215 culture grown on xylan detected by MALDI-TOF analysis

From: Identification of an endo-1,4-beta-xylanase of Ustilago maydis

Putative enzyme activity Gene(s) ScoreA Mw[kDa] pI Sequence coverage [%]A Number of peptidesA CAZy familyB Remark
endo-1,4-beta-xylanase um06350.1 87/111 23.8 9.41 24.0/18.1 4/3 GH11 xylan degradation
arabinoxylan arabinofurano-hydrolase um04309.1 149/142 36.3 9.00 21.1/13.6 5/3 GH62 xylan degradation
glycosidase um02727.1 179/180 25.1 9.27 26.7/26.7 3/3 GH25 D-glucan degradation
endo-1,3(4)-beta-glucanase um02134.1 331/58 41.5 5.84 17.3/2.3 4/1 GH16 D-glucan degradation
glycosyl hydrolase um06247.1 65/85 54.0 5.98 4.47/4.4 2/2   (hemi-) /cellulose degradation
chitin deacetylase um02689.1 -/100 52.4 6.63 -/6.0 -/2 CE4 polysaccharide degradation
lipase B um01422.1 118/130 35.1 8.81 8.3/8.3 2/2   lipid degradation
aspartic protease um00064.1 316/338 41.5 5.80 17.1/17.1 4/4   peptide degradation
alpha-galactosidase um04503.1 153/138 60.5 6.58 8.6/4.7 3/2 GH27 galactomannan degradation
spherulin 4 um06157.1 237/150 35.5 9.67 24.7/14.5 4/3   spherulin-like protein
choline dehydrogenase um03246.1 222/176 67.9 6.30 8.0/6.5 5/3   glycine, serine and threonine metabolism
uncharacterized protein um05604.1 88/101 27.0 4.65 6.0/6.0 3/3   unknown
uncharacterized protein um01894.1 49/48 30.4 5.53 6.5/6.5 2/2   unknown
uncharacterized protein um00961.1 367/371 31.6 6.19 24.8/20.7 4/3   unknown
  1. A: numbers are given for both analyses of a biological replicate.
  2. B: CAZy families are signed according to Cantarel et al. [47].