Figure 7
From: Diffusion of small molecules into medaka embryos improved by electroporation
Lithium induction causes deficiencies in anterior-posterior development. Embryos at 40% epiboly were exposed to 0.4 M LiCl for 10 minutes at 27°C. Subsequent electroporation was performed at 330 Hz; 15 V; 100 ms; 1 pulse. 24 hours after the induction (+24 hrs) the phenotypes were clearly detectable and finally categorized after 72 hours (+72 hrs) as: wild type (green; B, E), weak (blue; C, F) and strong phenotypes (red; D, G). (A) The control (Li+ ctrl) shows equally treated but non-electroporated embryos, electroporation control embryos (ELPO ctrl) were electroporated, but not exposed to LiCl. Electroporated embryos with LiCl (ELPO Li+) showed phenotypes indicated by colours. Embryos are shown in dorsal view with anterior to the top. Scale bar 100 μM.