Incubation with detergents. 3 day old embryos incubated for 40 minutes with fluorescein (10 mg/ml) at different time points of washing without addition (A-C; no Triton), or together with 0.03% Triton X-100 (D-F; + Triton). Embryos are shown in lateral view, anterior to the top. Note that pictures (A-C) were taken with 100 ms exposure time, whereas (D-F) with 10 ms. The accumulation of fluorescein in the liver/gallbladder at later stages can be seen in (C). Scale bar 250 μM.