Table 2 Oligonucleotide primers used in this study
Primer | 5’→3’ | REN |
|---|---|---|
TS_pCMVFc_Leader_f1 | CTGGCACGTGGAAATTAATTAAACGCCGCCAACATGGGATGGAGCTGT | PmlI |
TS_pCMVFc_NheI_r1 | AGATGCTAGCGGCCGCAGATCTGGGCCCGGATTCCTGCAGCTGGACCTGTGCCATGGAGTGCGCGCCTGTGGAGAGA | NheI, NcoI |
TS_hIgG1CH2_SacII_f | ACGGCGTGGAGGTGCATAATGCCAAGAC | |
TS_pCMV2.1_r | AGCTTCTAGACTCATTTACCCGGGGACAGGGAGA | XbaI |
TS_pCMV2.2_r | AGCTTCTAGACGCACTCATTTACCCGGGGACAGGGAGA | XbaI |
TS_pCMV2.3_r | AGCTTCTAGAGCCGGCCGTCGCACTCATTTACCCGGGGACAGGGAGA | XbaI |
TS_pCMV2.4_r | AGCTTCTAGATTCATTTACCCGGGGACAGGGAGA | XbaI |
TS_pCMV2.5_r | AGCTTCTAGATTTATTTACCCGGGGACAGGGAGA | XbaI |
TS_pCMV3.2_r | AGCTTCTAGACGCACTCATCCCGGGGACAGGGAGA | XbaI |
TS_pCMV4.2_r | AGCTTCTAGACGCACTCAGGATCCCGGGGACAGGGAGA | XbaI |
MHdCpCMV-NotI_f | GATCTGCGGCCGCTAGCGACAAAACTCACACATGCC | NotI |
MHCH2-SacII_r | CTCCTCCCGCGGCTTTGTCTTGG | SacII |
TS_oriP_PmeI-PvuII_f | GACTCAGCTGTTTAAACGAACTAAACCTGACTACG | PvuII, PmeI |
TS_oriP_FR_BsmBI/PciI_r | CACTCGTCTCACATGTATTCTCATGTTTGACAGC | BsmBI/PciI |
TS_pUCori_MfeI_f | CAGTCAATTGGGATATCGCAGGAAAGAGCATGTGAGC | MfeI, EcoRV |
TS_5’CMV_MfeI_r | CTGTCAATTGTGAATTCGATACCGATCTC | MfeI, EcoRI |
TS_oriPspacer_Pci_r | CAACACATGTCAACATTAGCCCACCGTGC | PciI |
TS_oriPspacer_Pml_f: | AGATCACGTGTAGGTGGGCGGGCCAAGA | PmlI |