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Table 1 pCMV-hIgG1Fc-XP vectors with modified 3′ translation termination sequences

From: High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

pCMV

5→3’

Features

-

TCTCCCTGTCCCCGGGTAAATGA GGATCCTCTAGAAGCT

opala, BamHI, XbaI

2.1

TCTCCCTGTCCCCGGGTAAATGA GTCTAGAAGCT

opal + 1 nt, XbaI

2.2

TCTCCCTGTCCCCGGGTAAATGA GTGCGTCTAGAAGCT

opal + 5 nts, XbaI

2.3

TCTCCCTGTCCCCGGGTAAATGA GTGCGACGGCCGGCTCTAGAAGCT

opal + 14 nts, XbaI

2.4

TCTCCCTGTCCCCGGGTAAATGA ATCTAGAAGCT

opal + dA, XbaI

2.5

TCTCCCTGTCCCCGGGTAAATAA ATCTAGAAGCT

ochre + dA, XbaI

3.2

TCTCCCTGTCCCCGGGATGA GTGCGTCTAGAAGCT

ΔK330b, opal + 5 nts, XbaI

4.2

TCTCCCTGTCCCCGGGATCCTGA GTGCGTCTAGAAGCT

K330Sb, c, BamHI, opal + 5 nts, XbaI

  1. Abreviations: nt, desoxynucleotide(s) derived from human IgG gene sequences; dA, desoxy-adenine.
  2. underlined … stop codon, bold letter… desoxynucleotide downstream from the stop codon also termed as “forth nucleotide of the stop codon” is important efficient for translation termination.
  3. a Frequent mutations of opal (TGA) to TTA were observed which might be due to the immediately following BamHI and XbaI sites.
  4. b Numbering according to Uniprot: P01857.
  5. c Additional BamHI fusion site.