| | On | Off | Induction | |
|---|
Construct | (rlu/μg) | | | Cells |
|---|
| | (x 107) | (x103) | (x103) | |
|---|
ES.1-Ptet-T6 | 4.1 ± 0.6 | 4.1 ± 0.1 | 9.8 ± 1.5 | HeLa-EM2 |
ES.1-scPtet-T6 | 1.9 ± 0.2 | 4.7 ± 0.6 | 4.0 ± 0.8 | HeLa-EM2 |
MOV-scT6 | 1.2 ± 0.01 | 9.2 ± 2.0 | 1.3 ± 0,3 | HeLa |
- Luciferase activity (rlu/μg protein) was determined after enrichment of transduced Hela-EM2 cells (ES.1 vectors) constitutively providing the M2 transactivator, or Hela cells (MOV-vector) in the presence (“on“) or absence (“off“) of doxycycline (500 ng/ml). Induction was calculated from the activities determined in the on and off state. ± values reflect SEM. For each vector, two populations were established by one round of sorting (pool purity >90%) and were measured twice.
