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Figure 7 | BMC Biotechnology

Figure 7

From: Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator

Figure 7

Induction kinetics of MOV-scT6 and MOV-scT6cA. (A) Luciferase activity of enriched Ht1080 cell populations transduced with MOV-T6sc or MOV-scT6cA vectors. Doxycycline concentration (1000 ng/ml) was kept constant during the experiment by daily medium exchange. Values represent data from two independently generated populations. All measurements were accomplished in duplicate. (B) One representative population is shown for a parallel determination of GFP fluorescence in FACS. The M1-region was used for the determination of the percentage of GFP positive cells. (C) Induced cells (reaching M1-region in “B“). Mean values of two independently generated populations. All measurements were done as duplicates.

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BMC Biotechnology

ISSN: 1472-6750

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