Figure 4

Dose response kinetic. (A) Luciferase activity of enriched Ht1080 cell populations transduced with MOV-scT6 or MOV-scT6cA vectors. Doxycycline concentrations were kept constant for four days by daily medium exchange. Values represent data from two independently generated populations. All measurements were accomplished in duplicate. (B) One representative population was used for a parallel determination of GFP fluorescence by flow cytometry. The percent GFP-positive cells (x-axis) are given within each blot measured against propidium iodide (PI, 1 μg/ml) stained dead cells (y-axis). It should be noted, that the purity of the enriched populations differed slightly. (C) Percent positive cells of the two independently generated populations. All measurements were accomplished in duplicate.