Figure 4
From: Generation of a tumor- and tissue-specific episomal non-viral vector system
Transient transfection efficiency and establishment efficiency of episomes in HEK293 and TE-671. Cells were transfected with the indicated plasmid and Fugene 6 as transfection reagent (A) Percentage of EGFP positive cells 48 hours after transfection; n = 6 + stddev, ** p = 0.003 (U-test, Mann–Whitney, pEPito-hCMV/SM22-IRES-BSD transfection HEK293 vs. TE-671) (B) Relative colony-forming efficiency of stably selected clones normalized to the establishment efficiency obtained with (pEPito-1- [hCMV/EF1αP]- [EGFP-IRES-BSD]-ΔSMAR). n = 6 + stddev.