Figure 2
From: Generation of a tumor- and tissue-specific episomal non-viral vector system
Expression activity of CMV and AFP promoter driven plasmids in human and murine cell lines. Indicated cell lines were seeded in 96-well plates (10,000 cells/well seeded), transfected with LPEI polyplexes (200 ng Plasmid/well) for four hours and the luciferase activity per 10,000 cells measured 24 h thereafter (n = 5 + stddev) (A) Absolute luciferase activity (RLU). Background activity of untransfected cells was below 1000 RLU for all cell lines evaluated. (B) Relative luciferase activity of plasmids pEPito-AFP-EGFPLuc and pEPito-hCMV/AFP-EGFPLuc normalized to the expression activity of pEPito-CMV-EGFPLuc in the respective cell line; insert in (B): mRNA level of AFP mRNA normalized to the housekeeper mRNA GAPDH in indicated cell lines (n = 2 + stddev., n.d.: not detectable).