Table 2 Oligonucleotide primers used in this study
Name | Sequence |
|---|---|
A-up | 5’-GGAATTCCATATGGGGCAATCACACACGC-3’ |
P-down | 5’-CCGGAATTCTCAAGCCATTGCGGCAACGA-3’ |
B-down(rbs) | 5’-ATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTC-3’ |
P-up(rbs) | 5’-TGAAAGGAGATATAGATATGAAAGACGAACGGTTT-3’ |
P-up | 5’-GGAATTCCATATGAAAGACGAACGGTTT-3’ |
P-down(PAB) | 5’-CATATCTATATCTCCTTTCAAGCCATTGCGGCAACG-3’ |
A-up(PAB) | 5’-TGAAAGGAGATATAGATATGGGGCAATCACACACGC-3’ |
B-down(PAB) | 5’-CCGGAATTCTCACGCTGGCTCCAGGTAGT-3’ |
Po-up | 5’-GGAATTCCATATGAAAGACGAACGTTTC-3’ |
Po-down | 5’-CCGGAATTCTCAAGCCATAGCAGCAACAA-3’ |
B-down(strep) | 5’-GCGGGTGGCTCCAGCTTGCCATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3’ |
P-up(strep) | 5’-AAGCTGGAGCCACCCGCAGTTCGAAAAGGGTGCAAAAGACGAACGGTTTCCATT-3’ |
K2A-up | 5’-TGAAAGGAGATATAGATATGGCGGACGAACGGTTTC-3’ |
K2A-down | 5’-GCAATGGAAACCGTTCGTCCGCCATATCTATATCTCC-3’ |
P-up(△N) | 5’-TGAAAGGAGATATAGATATGGGCCCTGTGTTTGACG-3’ |
A-down(strep) | 5’-GCGGGTGGCTCCAGCTTGCCATATCTATATCTCCTTTTAATGAGATGGGGTGGGTTGGGT-3’ |
secA-up | 5’-CCGGAATTCGGGGCAATCACACACGCAT-3’ |
secB-down | 5’-GATATCCATGGCCATCGCCGGCTGGGCAGCGAGGAGCAGCAGACCAGCAGCAGCGGTCGGCAGCAGGTATTTCATATGTATATCTCCTTTCACGCTGGC-3’ |
secP-up | 5’-CAGCCGGCGATGGCCATGGATATCGGAATTAATGCAAGCTGGAGCCACCCGCAGTTCGAAAAGGGTGCAAAAGACGAACGGTTTCCATTGCCAG-3’ |
secP-down | 5’-CGACCCAAGCTTTCAAGCCATTGCGGCAACGACC-3’ |