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Figure 5 | BMC Biotechnology

Figure 5

From: Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

Figure 5

SDS-PAGE analysis of recombinant pelB-strep-P14K. (A) SDS-PAGE analysis of the activators encoded by A(strep-P) and (pelB-A)B(pelB-strep-P). 1, mark; 2, cell extracts of the transformant containing pET-A(strep-P); 3, cell extracts of the transformant containing pET-(pelB-A)B(pelB-strep-P); 4, culture supernatant of the transformant containing pET-(pelB-A)B(pelB-strep-P). (B) SDS-PAGE analysis of stability of purified activator complexes [α-(strep-P14K)] and [α-(pelB-strep-P14K)]. 1, mark; 2, purified [α-(strep-P14K)]; 3, purified [α-(strep-P14K)] after storage for 1 day; 4, purified [α-(strep-P14K)] after storage for 2 days; 5, purified [α-(pelB-strep-P14K)]; 6, purified [α-(pelB-strep-P14K)] after storage for 2 days; 7, purified [α-(pelB-strep-P14K)] after storage for 6 days.

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