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Figure 4 | BMC Biotechnology

Figure 4

From: Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

Figure 4

SDS-PAGE analysis of recombinant NHases and P14K, and the predicted secondary structure of P14K. (A) SDS-PAGE analysis of the cell extracts of the transformants carrying pET-ABP, pET-AB’P, pET-PAB or pET-ABPo. 1, mark; 2, pET-ABP; 3, pET-AB’P; 4, pET-PAB; 5, pET-ABPo. (B) Predicted secondary structure of P14K. (C) SDS-PAGE analysis of the cell extracts of the transformants carrying pET-AB(strep-P), pET-ABP(K2A) or pET-AB(N-P). 1, mark; 2, pET-ABP; 3, pET-AB(strep-P); 4, pET-ABP(K2A); 5, harboring pET-AB(N-P).

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