Figure 3

Sketch of the plasmids used in this study. pET-ABP, a plasmid containing the ABP genes; pET-AB’P, a plasmid containing the ABP genes with an enhanced RBS (AAGGAG) between the B and P genes; pET-PAB, a plasmid containing the ABP genes with a < P > <A > <B > order; pET-ABPo, a plasmid containing the AB and the codon-optimized P genes; pET-AB(strep-P), a plasmid containing the AB and a strep-tagged P genes [18]; pET-ABP(K2A), a plasmid harboring the AB and a mutant P (the second lysine (K) is mutated to alanine (A)) genes; pET-AB(^△N-P), a plasmid harboring the AB and a 16 amino-acid truncated N terminus P genes; pET-A(strep-P), a plasmid containing the A and a strep-tagged P genes; pET-(pelB-A)B(pelB-strep-P), a pET-22b plasmid containing the pelB-A, B and pelB-strep-P genes.