Table 4 Strains and plasmids used in this study
From: Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine
Strain, plasmid | Properties/sequence | Source/reference |
|---|---|---|
Strain | Â | Â |
Escherichia coli DH5α | supE44, hsdR17, recA1, thi-1, endA1, lacZ, gyrA96, relA1 | Invitrogen |
C. glutamicum | Â | Â |
ATCC 13032 | Wild-type | ATCC |
ATCC 13032 (ΔargFΔproB) | C. glutamicum ATCC 13032, ΔargF, ΔproB | 15 |
ΔAPE | C. glutamicum ATCC 13032, ΔargF, ΔproB, ΔspeE | This study |
ΔAPE6937 | The evolved strain of ATCC 13032 (ΔargFΔproBΔspeE) | This study |
ΔAPE6937R42 | ΔAPE6937, ΔargR | This study |
ΔAPER | C. glutamicum ATCC 13032, ΔargF, ΔproB, ΔspeE, ΔargR | This study |
pK18mobsacB | sacB, lacZa, Kmr, mcs mobilizable vector, allows for selection of double crossover C. glutamicum | 39 |
pK-JL | pK18mobsacB derivative, sacB under the control of tac-M promoter, Kmr, | This study |
pMD18-T | TA cloning vector, Ampr | TaKaRa |
pK-ΔargR | pK-JL with 506 bp deletion of the argR gene | This study |
pEC-XK99E | C. glutamicum-E. coli shuttle expression vector, Kanr | 41 |
pEC-argBCG | pEC-XK99E containing the argB gene from C. glutamicum | This study |
pEC-argBEC | pEC-XK99E containing the argB gene from E. coli | This study |
pEC-zwf | pEC-XK99E containing the zwf gene from C. glutamicum | This study |
pEC-ppnK | pEC-XK99E containing the ppnk gene from C. glutamicum | This study |
pEC-gnd | pEC-XK99E containing the gnd gene from C. glutamicum | This study |
pEC-pntAB | pEC-XK99E containing the gnd gene from E. coli | This study |