Figure 4

Affinity chromatography on a chitin matrix (A) and SDS-PAGE analysis (B). (A) Purification of recombinant CvChi45 secreted into the E. coli culture medium. The secreted proteins were concentrated as described in the Methods section and loaded onto a chitin column that was equilibrated with NaAc buffer (pH 5.2) containing 1 M NaCl. After washing off the unbound proteins (P1), the recombinant protein was eluted with 0.1 M acetic acid (P2). (B) SDS-PAGE analysis of purified recombinant CvChi45 (lane 1). M: molecular weight markers.