Figure 3

Detection of Cv Chi45 in protein fractions of E . coli by SDS-PAGE (A) and Western blotting (B). Protein fractions (soluble intracellular extract, I; periplasm extract, P; and cell-free medium supernatant, S) from E. coli cells transformed with pET303/CT-His or pET-CV2935 and induced under the same conditions were prepared as described in the Methods section. Proteins (100 μg per lane) were resolved by SDS-PAGE and stained with Coomassie Brilliant Blue (A) or transferred to a nitrocellulose membrane and submitted to immunodetection using an anti-His tag antibody (B), as described in the Methods section. The protein band corresponding to rCvChi45 is indicated by an arrow. M: molecular weight markers.