Figure 1From: Optimization and validation of mitochondria-based functional assay as a useful tool to identify BH3-like molecules selectively targeting anti-apoptotic Bcl-2 proteinsBreast cancer cells are dually or multiply-protected by anti-apoptotic Bcl-2 family proteins. Cell lines were cultured under conditions recommended by ATCC. Whole cell lysates were subjected to SDS-PAGE and Western blotting analysis for the basal level of three major anti-apoptotic Bcl-2 family proteins, Bcl-2, Bcl-xL and Mcl-1.Back to article page