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Table 2 Simulation parameter sets for cultivations in medium (1) and (2) without and with additional salts

From: In situ magnetic separation of antibody fragments from Escherichia coli in complex media

 

k tr [m/h]

k d [g/L]

Q max [g/g]

A spec [m2/g]

r P,i,0 [mg g-1 h-1]

r P,j,0 [g g-1 h-1]

r P,i,deg [h-1]

r cys [mg g-1 h-1]

K5/K7/K8

2.5e-06 a1)

0.039 a2)

0.081 a2)

44 b)

0; 1-6 c)

0.003 (t > 0) c)

0 d)

0.2 after ~40 h c)

K9/K10/K11

5.95e-05 a1)

0.014 a2)

0.1 a2)

44 b)

0; 1 c)

0.003 (t > 0) c)

0 d)

0.0 c)

All cultivations

μ max [1/h]

r m [g g-1h-1]

m f [−]

q feed [g/h]

q sampler [mL/h]

K M,glu [g/L]

K M,aa (cys) [g/L]

Y X,glu [g/g]

 

0.37-0.44 a3)

0.2 c)

0.5-0.7 b)

2-4 e)

12.5 e)

0.1 b)

0.01 b)

0.25 d)

 

Y X,aa (cys) [g/g]

Y CO2,X [g/g]

Y CO2,P,i (j) [g/g]

e C,glu [−]

e C,CO2 [−]

e C,X [−]

e C,P,i (j) [−]

e aa,X [−]

 

1.5 (15) c)

0.45 b)

0.5 b)

0.4

0.27

0.5 b)

0.43 b)

0.64 b)

 

e aa,P,i(j) [−]

e cys,yeast [−]

e cys,X [−]

e cys,P,i [−]

e cys,P,j [−]

E a [J/mol]

A Arrh. [1/h]

 
 

1.0 c)

0.0073 b)

0.012 b)

0.028 b)

0 c)

56000 b)

1.2e9 c)

 
  1. a) estimated by minimizing least squares for Eqs. a1) 3-7, a2) 2-1, a3) values valid for 37°C; for 25°C calculation according to Equation 3-10.
  2. b) estimated from literature.
  3. c) estimated manually.
  4. d) according to measurements.
  5. e) set manually.