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Table 2 Simulation parameter sets for cultivations in medium (1) and (2) without and with additional salts

From: In situ magnetic separation of antibody fragments from Escherichia coli in complex media

  k tr [m/h] k d [g/L] Q max [g/g] A spec [m2/g] r P,i,0 [mg g-1 h-1] r P,j,0 [g g-1 h-1] r P,i,deg [h-1] r cys [mg g-1 h-1]
K5/K7/K8 2.5e-06 a1) 0.039 a2) 0.081 a2) 44 b) 0; 1-6 c) 0.003 (t > 0) c) 0 d) 0.2 after ~40 h c)
K9/K10/K11 5.95e-05 a1) 0.014 a2) 0.1 a2) 44 b) 0; 1 c) 0.003 (t > 0) c) 0 d) 0.0 c)
All cultivations μ max [1/h] r m [g g-1h-1] m f [−] q feed [g/h] q sampler [mL/h] K M,glu [g/L] K M,aa (cys) [g/L] Y X,glu [g/g]
  0.37-0.44 a3) 0.2 c) 0.5-0.7 b) 2-4 e) 12.5 e) 0.1 b) 0.01 b) 0.25 d)
  Y X,aa (cys) [g/g] Y CO2,X [g/g] Y CO2,P,i (j) [g/g] e C,glu [−] e C,CO2 [−] e C,X [−] e C,P,i (j) [−] e aa,X [−]
  1.5 (15) c) 0.45 b) 0.5 b) 0.4 0.27 0.5 b) 0.43 b) 0.64 b)
  e aa,P,i(j) [−] e cys,yeast [−] e cys,X [−] e cys,P,i [−] e cys,P,j [−] E a [J/mol] A Arrh. [1/h]  
  1.0 c) 0.0073 b) 0.012 b) 0.028 b) 0 c) 56000 b) 1.2e9 c)  
  1. a) estimated by minimizing least squares for Eqs. a1) 3-7, a2) 2-1, a3) values valid for 37°C; for 25°C calculation according to Equation 3-10.
  2. b) estimated from literature.
  3. c) estimated manually.
  4. d) according to measurements.
  5. e) set manually.