From: In situ magnetic separation of antibody fragments from Escherichia coli in complex media
k_{ tr }[m/h] | k_{ d }[g/L] | Q_{ max }[g/g] | A_{ spec }[m^{2}/g] | r_{ P,i,0 }[mg g^{-1} h^{-1}] | r_{ P,j,0 }[g g^{-1} h^{-1}] | r_{ P,i,deg }[h^{-1}] | r_{ cys }[mg g^{-1} h^{-1}] | |
---|---|---|---|---|---|---|---|---|
K5/K7/K8 | 2.5e-06 ^{a1)} | 0.039 ^{a2)} | 0.081 ^{a2)} | 44 ^{b)} | 0; 1-6 ^{c)} | 0.003 (t > 0) ^{c)} | 0 ^{d)} | 0.2 after ~40 h ^{c)} |
K9/K10/K11 | 5.95e-05 ^{a1)} | 0.014 ^{a2)} | 0.1 ^{a2)} | 44 ^{b)} | 0; 1 ^{c)} | 0.003 (t > 0) ^{c)} | 0 ^{d)} | 0.0 ^{c)} |
All cultivations | μ _{ max } [1/h] | r_{ m }[g g^{-1} h^{-1}] | m _{ f } [−] | q _{ feed } [g/h] | q _{ sampler } [mL/h] | K _{ M,glu } [g/L] | K _{ M,aa (cys) } [g/L] | Y _{ X,glu } [g/g] |
0.37-0.44 ^{a3)} | 0.2 ^{c)} | 0.5-0.7 ^{b)} | 2-4 ^{e)} | 12.5 ^{e)} | 0.1 ^{b)} | 0.01 ^{b)} | 0.25 ^{d)} | |
Y _{ X,aa (cys) } [g/g] | Y _{ CO2,X } [g/g] | Y _{ CO2,P,i (j) } [g/g] | e _{ C,glu } [−] | e _{ C,CO2 } [−] | e _{ C,X } [−] | e _{ C,P,i (j) } [−] | e _{ aa,X } [−] | |
1.5 (15) ^{c)} | 0.45 ^{b)} | 0.5 ^{b)} | 0.4 | 0.27 | 0.5 ^{b)} | 0.43 ^{b)} | 0.64 ^{b)} | |
e _{ aa,P,i(j) } [−] | e _{ cys,yeast } [−] | e _{ cys,X } [−] | e _{ cys,P,i } [−] | e _{ cys,P,j } [−] | E _{ a } [J/mol] | A _{ Arrh. } [1/h] | ||
1.0 ^{c)} | 0.0073 ^{b)} | 0.012 ^{b)} | 0.028 ^{b)} | 0 ^{c)} | 56000 ^{b)} | 1.2e9 ^{c)} |