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Table 1 Overview of cultivation and separation conditions

From: In situ magnetic separation of antibody fragments from Escherichia coli in complex media

Cultivation K4 K5 K7 K8 K9 K10 K11 SK1 SK2 SK3
Medium (2) (2) (2) (2) (1) (1) (1) (3) (1) (3)
t IPTG,ΔT [h] 11 11 10 10 10 13 12 3.5 8.5 8.5
t ISMS [h] - - 75/119 78 - - 54 8.5 12.3 12.3
Ligand - - IDA-2 IDA-2 - - IDA-1 IDA-1 triazine 22/8  
m mp [g] - - 28 28 - - 8 0.2 0.4 0.4
  1. K4 was conducted using the same cultivation parameters and medium as in K5 (results not shown).
  2. Shaking flask cultivations SK1 and SK 3 were performed in medium (3) containing tryptone instead of casamino acids, but 0.2 g IDA-1 beads were applied in SK1 while triazine beads were used in SK3.