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Table 8 Gene constructs for additive gene transfer

From: Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

Gene Promoter Coding sequence 3’-UTR/pA
hTM 8.9 kb poTM 1.9 kb huTM gene 0.3 kb boGH
CAG-Case12 1.7 kb CAG [61]§ 1.2 kb Case12a 0.6 kb raHBB [61]§
CAG-TA [8]§ 1.7 kb CAG [61]§ 1.0 kb rtTAb 0.3 kb boGH
CAG-LEA 1.7 kb CAG [61]§ 1.2 kb LEA29Yc 0.6 kb raHBB [61]§
INS-LEA [62]§ 1.5 kb po INS 1.2 kb LEA29Yc 0.3 kb boGH
INS-C94Y [16]§ 2.5 kb po INS fragment including point mutation
INS-C93S 2.5 kb po INS fragment including point mutation
INS-TK 1.5 kb po INS 1.1 kb TKc 0.3 kb boGH
COL-TK 3.6 kb po COL1A1 1.1 kb TKc 0.3 kb boGH
CFTR-LacZ CH242-248P18 3.5 kb lacZd 0.3 kb boGH
GGTA-LacZ CH242-21 F3 3.5 kb lacZd 0.3 kb boGH
TRE-RANKL [8]§ 0.3 kb TREa 0.9 kb po sRANKL 0.3 kb boGH
TRE-CTLA-4Ig [8]§ 0.3 kb TREa 1.2 kb po CTLA4-Ig 0.3 kb boGH
HAC1 [63]§ 0.6 kb CMV 0.7 kb GFP 0.3 kb SV40
  1. § See indicated references.
  2. a Purchased from Evrogen, Moscow, Russia.
  3. b Purchased from Clontech, Mountain View, CA.
  4. c Custom-synthesized by Bio&Sell, Feucht, Germany.
  5. d Purchased from Promega, Madison, WI.