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Table 2 Stratification and data distribution of the investigated experimental factors

From: Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

Factor No. of embryo transfers (%)
Season 1   
- Spring 39 (20.2)
- Summer 59 (30.6)
- Autumn 58 (30.0)
- Winter 37 (19.2)
Type of genetic modification 2   
- de novo - AGT 57 (29.5)
- HR 48 (24.9)
- replication of transgenic pig 88 (45.6)
Donor cell source 3   
- MSC 36 (18.7)
- PF 24 (12.4)
- FF 51 (26.4)
- KC 82 (42.5)
Cloning rounds   
- 1 time 110 (57.0)
- 2 times 62 (32.1)
- 3 times 21 (10.9)
Selection of SCNT embryos for early development 4   
- no selection 45 (23.3)
- selection after 1 day 13 (6.7)
- selection after 2 days 15 (7.8)
- mixed selection 120 (62.2)
  1. Additional file 3 and Additional file 4 show in more detail the distribution in season and embryo selection of specific SCNT configurations with respect to genetic modification, donor cell source and number of cloning rounds.
  2. 1 Embryo transfer date.
  3. 2 AGT: additive gene transfer, HR: homologous recombination.
  4. 3 Mesenchymal stem cells (MSC), postnatal fibroblasts (PF), fetal fibroblasts (FF), and kidney cells (KC).
  5. 4 No selection: all SCNT embryos transferred, selection for 1 day: 1-cell stage SCNT embryos transferred, selection for 2 days: 2-cell to 4-cell stage SCNT embryos transferred, mixed selection: mixed SCNT embryos transferred (no selection/1 day and 1 day/2 days).