|
Factor
|
No. of embryo transfers (%)
|
|---|
|
Season
1
| | |
|
- Spring
|
39
|
(20.2)
|
|
- Summer
|
59
|
(30.6)
|
|
- Autumn
|
58
|
(30.0)
|
|
- Winter
|
37
|
(19.2)
|
|
Type of genetic modification
2
| | |
|
- de novo - AGT
|
57
|
(29.5)
|
|
- HR
|
48
|
(24.9)
|
|
- replication of transgenic pig
|
88
|
(45.6)
|
|
Donor cell source
3
| | |
|
- MSC
|
36
|
(18.7)
|
|
- PF
|
24
|
(12.4)
|
|
- FF
|
51
|
(26.4)
|
|
- KC
|
82
|
(42.5)
|
|
Cloning rounds
| | |
|
- 1 time
|
110
|
(57.0)
|
|
- 2 times
|
62
|
(32.1)
|
|
- 3 times
|
21
|
(10.9)
|
|
Selection of SCNT embryos for early development
4
| | |
|
- no selection
|
45
|
(23.3)
|
|
- selection after 1 day
|
13
|
(6.7)
|
|
- selection after 2 days
|
15
|
(7.8)
|
|
- mixed selection
|
120
|
(62.2)
|
- Additional file 3 and Additional file 4 show in more detail the distribution in season and embryo selection of specific SCNT configurations with respect to genetic modification, donor cell source and number of cloning rounds.
- 1 Embryo transfer date.
- 2 AGT: additive gene transfer, HR: homologous recombination.
- 3 Mesenchymal stem cells (MSC), postnatal fibroblasts (PF), fetal fibroblasts (FF), and kidney cells (KC).
- 4 No selection: all SCNT embryos transferred, selection for 1 day: 1-cell stage SCNT embryos transferred, selection for 2 days: 2-cell to 4-cell stage SCNT embryos transferred, mixed selection: mixed SCNT embryos transferred (no selection/1 day and 1 day/2 days).
