Crystalline cellulose hydrolysis by cellulases with a POSWOI-pretreated procedure. Purified POSWOI (10 μg) was first incubated with 2.5 mg of Avicel at 50°C for 48 h. The mixture was incubated at 90°C for 10 min to inactivate the POSWOI, and then rinsed with sodium acetate buffer to remove the POSWOI. Cellulases (0.002 FPU) were mixed with the pretreated Avicel in 250 μL 0.05 mol/L sodium acetate buffer at 50°C. Samples were collected at 0, 4, 8, 12, 24, 36, 48 h, respectively and subjected to reducing sugar evaluation by dinitrosalicylic acid (DNS) reagent. The Avicel pretreated by sodium acetate buffer only was used as a control. Each reaction was performed in triplicate, and data points and error bars indicate the mean ± standard deviations.