Figure 4

Synergism between POSWOI and cellulases. Different quantities of purified POSWOI (0.3, 3, 10, 30 μg) and a fixed quantity of cellulases (0.002 FPU) were incubated with 2.5 mg of Avicel in 250 μL of 0.05 mol/L sodium acetate buffer at 50°C. Samples were collected at 0, 4, 8, 12, 24, 36, 48 h, respectively, and subjected to reducing sugar evaluation by dinitrosalicylic acid (DNS) reagent. The reaction containing cellulases or POSWOI alone served as a control. Each reaction was performed in triplicate, and the data points and error bars indicate the mean ± standard deviations.