Production and purification of recombinant POSWOI in T. reesei RUT-C30. A: SDS-PAGE analysis of the culture supernatant of six poswo1 transformants. Lane 1: parental strain T. reesei RUT-C30; Lane 2 ~ 7: poswo1 transformants of POS-7, POS-10, POS-12, POS-13, POS-17, and POS-20. The quantity of the CBHI and POSWOI bands was evaluated by density scan. B: Western blot analysis of the same samples of A. C: SDS-PAGE analysis of various protein fractions collected during recombinant POSWOI purification. Lane 1: POSWOI transformant culture supernatant (Raw); Lane 2: POSWOI fraction collected after ultrafiltration (UF); Lane 3: POSWOI fraction collected after ammonium sulfate precipitation (SA); Lane 4: POSWOI fraction eluted in immobilized metal affinity chromatography (IMAC). Arrows show the recombinant POSWOI and native CBHI.