Figure 8
Effect of p19 suppressor of gene silencing on transgenic tobacco lines with low accumulation levels. A. Quantification of GFP expression in infiltrated tissue of young and old leaves. Light blue represents the mock-infiltrated area with Gamborg’s solution (control). Dark blue represents the p19 infiltrated tissue. Error bars represent technical replicates of GFP quantitation in agro-infiltrated sectors. Absence of bars indicates no GFP detection B. UV visualization of p19-infiltrated and mock-infiltrated tissues at 2 dpi in young leaves. The p19-infiltrated sectors (right) fluoresce green compared to the mock-infiltrated sectors (left). C. Daylight visualization of p19-infiltrated and mock-infiltrated young and old leaves at 5 dpi; slight chlorosis is apparent in young leaves only. Infiltrated areas are delineated with dotted black lines.