Figure 3

Xylanase inhibition assay of OsCLP. A, xylanase inhibition by OsCLP on agar plate containing xylan, as evidenced by the presence of a xylan degradation circle. Inhibition of xylanases isolated from A. pullulans and T. viride by OsCLP were performed on a 1.5% LB agar plate containing 1% (w/v) birchwood xylan substrate. B, analysis of the degree of xylanase inhibition by OsCLP. Samples included 10 μmol boiled xylanase (A. pullulans), 10 μmol xylanase (A. pullulans) with chemical inhibitor (15 mM N-bromosuccinimide), 5 μg BSA with 10 μmol xylanase (T. viride), 10 μmol xylanase (T. viride) only, 10 μg OsCLP with 10 μmol xylanase (T. viride), 10 μmol xylanase (A. pullulans) only, and 10 μg OsCLP with 10 μmol xylanase (A. pullulans). All statistical analyses were performed using Student’s t test (P < 0.05). Dashed circles indicate positions of paper disks.